Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo.

ABSTRACT: Coxsackievirus belongs to the Picornaviridae family and is one of the major pathogens that cause hand, foot and mouth disease (HFMD) in infants and children with potential serious complications and even deaths. The pathogenesis of this virus is not fully elucidated and no vaccine or antiviral drug has been approved. In this study, a full-length infectious cDNA clone of coxsackievirus B5 virus was assembled and the recombinant virus displayed similar growth kinetics and ability to cause cytopathic effects as the parental virus. Luciferase reporter was then incorporated to generate both full-length and subgenomic replicon (SGR) reporter viruses. The full-length reporter virus is suitable for high-throughput antiviral screening, while the SGR is a useful tool to study viral-host interactions.

SUBMITTER: Guo S 

PROVIDER: S-EPMC10436053 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature