X-ray Structure Characterization of the Selective Recognition of AT Base Pair Sequences.

ABSTRACT: The rational design of small molecules that target specific DNA sequences is a promising strategy to modulate gene expression. This report focuses on a diamidinobenzimidazole compound, whose selective binding to the minor groove of AT DNA sequences holds broad significance in the molecular recognition of AT-rich human promoter sequences. The objective of this study is to provide a more detailed and systematized understanding, at an atomic level, of the molecular recognition mechanism of different AT-specific sequences by a rationally designed minor groove binder. The specialized method of X-ray crystallography was utilized to investigate how the sequence-dependent recognition properties in general, A-tract, and alternating AT sequences affect the binding of diamidinobenzimidazole in the DN

SUBMITTER: Ogbonna EN 

PROVIDER: S-EPMC10436263 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature