Chemoenzymatic Syntheses of Fluorine-18-Labeled Disaccharides from [¹⁸F] FDG Yield Potent Sensors of Living Bacteria In Vivo.

ABSTRACT: Chemoenzymatic techniques have been applied extensively to pharmaceutical development, most effectively when routine synthetic methods fail. The regioselective and stereoselective construction of structurally complex glycans is an elegant application of this approach that is seldom applied to positron emission tomography (PET) tracers. We sought a method to dimerize 2-deoxy-[¹⁸F]-fluoro-d-glucose ([¹⁸F]FDG), the most common tracer used in clinical imaging, to form [¹⁸F]-labeled disaccharides for detecting microorganisms in vivo based on their bacteria-specific glycan incorporation. When [¹⁸F]FDG was reacted with β-d-glucose-1-phosphate in the presence of maltose phosphorylase, the α-1,4- and α-1,3-linked products 2-deoxy-[¹⁸F]-f

SUBMITTER: Sorlin AM 

PROVIDER: S-EPMC10436271 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature