Strategies for Overcoming the Single-Molecule Concentration Barrier.

ABSTRACT: Fluorescence-based single-molecule approaches have helped revolutionize our understanding of chemical and biological mechanisms. Unfortunately, these methods are only suitable at low concentrations of fluorescent molecules so that single fluorescent species of interest can be successfully resolved beyond background signal. The application of these techniques has therefore been limited to high-affinity interactions despite most biological and chemical processes occurring at much higher reactant concentrations. Fortunately, recent methodological advances have demonstrated that this concentration barrier can indeed be broken, with techniques reaching concentrations as high as 1 mM. The goal of this Review is to discuss the challenges in performing single-molecule fluorescence techniques at hi

SUBMITTER: White DS 

PROVIDER: S-EPMC10436376 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature