Rutaecarpine induces the differentiation of triple-negative breast cancer cells through inhibiting fumarate hydratase.

ABSTRACT:

Background

Triple-negative breast cancer (TNBC) is one of the most aggressive human cancers and has poor prognosis. Approximately 80% of TNBC cases belong to the molecular basal-like subtype, which can be exploited therapeutically by inducing differentiation. However, the strategies for inducing the differentiation of TNBC remain underexplored.

Methods

A three-dimensional (3D) morphological screening model based on a natural compound library was used to identify possible candidate compounds that can induce TNBC cell differentiation. The efficacy of rutaecarpine was verified using assays: RT-qPCR, RNA-seq, flow cytometry, immunofluorescence, SCENITH and label-free LC-MS/MS. The direct targets of rutaecarpine were identified through drug affinity responsive target stability (D

SUBMITTER: Lei J 

PROVIDER: S-EPMC10436383 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature