Use of viability PCR for detection of live Chlamydia trachomatis in clinical specimens.

ABSTRACT:

Background

The current testing approach to diagnose Chlamydia trachomatis (CT) infection relies on nucleic acid amplification tests (NAATs). These tests are highly sensitive, but do not distinguish between active infection and residual bacterial nucleic acid which may remain after resolution of infection, or via cross-contamination. Better methods to assess the viability of CT detected in clinical samples would be useful in determining the relevance of CT detection in a variety of clinical settings. The goal of this study was to test viability PCR (vPCR) as a method to distinguish viable bacteria from non-viable CT.

Methods

The vPCR relies on a propidium monoazide dye (PMAxx), which intercalates into accessible DNA from dead organisms and prevents their detection in a

SUBMITTER: Vojtech L 

PROVIDER: S-EPMC10436598 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature