The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum.

ABSTRACT:

Background

During the production of L-arginine through high dissolved oxygen and nitrogen supply fermentation, the industrial workhorse Corynebacterium glutamicum is exposed to oxidative stress. This generates reactive oxygen species (ROS) and reactive nitrogen species (RNS), which are harmful to the bacteria. To address the issue and to maintain redox homeostasis during fermentation, the flavohaemoprotein (Hmp) was employed.

Results

The results showed that the overexpression of Hmp led to a decrease in ROS and RNS content by 9.4% and 22.7%, respectively, and improved the survivability of strains. When the strains were treated with H₂O₂ and NaNO₂, the RT-qPCR analysis indicated an up-regulation of ammonium absorption and transporter genes amt

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC10436651 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature