An efficient method for high molecular weight bacterial DNA extraction suitable for shotgun metagenomics from skin swabs.

ABSTRACT: The human skin microbiome represents a variety of complex microbial ecosystems that play a key role in host health. Molecular methods to study these communities have been developed but have been largely limited to low-throughput quantification and short amplicon-based sequencing, providing limited functional information about the communities present. Shotgun metagenomic sequencing has emerged as a preferred method for microbiome studies as it provides more comprehensive information about the species/strains present in a niche and the genes they encode. However, the relatively low bacterial biomass of skin, in comparison to other areas such as the gut microbiome, makes obtaining sufficient DNA for shotgun metagenomic sequencing challenging. Here we describe an optimised high-throughput meth

SUBMITTER: Serghiou IR 

PROVIDER: S-EPMC10438817 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature