Novel characterization of CXCR4 expressing cells in uninfected and herpes simplex virus-1 infected corneas.

ABSTRACT:

Purpose

To characterize CXCR4-expressing cells in uninfected and herpes simplex virus-1 (HSV-1) infected corneas.

Methods

The corneas of C57BL/6J mice were infected with HSV-1 McKrae. The RT-qPCR assay detected CXCR4 and CXCL12 transcripts in uninfected and HSV-1-infected corneas. Immunofluorescence staining for CXCR4 and CXCL12 protein was performed in the frozen sections of herpes stromal keratitis (HSK) corneas. Flow cytometry assay characterized the CXCR4-expressing cells in uninfected and HSV-1-infected corneas.

Results

Flow cytometry data showed CXCR4 expressing cells in the separated epithelium and stroma of uninfected corneas. In the uninfected stroma, CD11b + F4/80+ macrophages are the predominant CXCR4-expressing cells. In contrast, most CXCR4 expressing cel

SUBMITTER: Suvas PK 

PROVIDER: S-EPMC10439974 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature