Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates.

ABSTRACT: Adeno-associated viruses (AAVs) are the vector of choice for delivering gene therapies that can cure inherited and acquired diseases. Clinical research on various AAV serotypes significantly increased in recent years alongside regulatory approvals of AAV-based therapies. The current AAV purification platform hinges on the capture step, for which several affinity resins are commercially available. These adsorbents rely on protein ligands-typically camelid antibodies-that provide high binding capacity and selectivity, but suffer from low biochemical stability and high cost, and impose harsh elution conditions (pH < 3) that can harm the transduction activity of recovered AAVs. Addressing these challenges, this study introduces peptide ligands that selectively capture AAVs and release them und

SUBMITTER: Chu W 

PROVIDER: S-EPMC10440015 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature