Effect of melatonin on steroidogenesis-related enzymes expression and testosterone synthesis following CoCl₂-induced hypoxia in TM3 Leydig cells.

ABSTRACT:

Objectives

This study examined the effects of melatonin treatment on steroidogenesis dysfunction and testosterone impairment, following CoCl₂-induced hypoxia in TM3 Leydig cells.

Materials and methods

The TM3 cells were divided into four groups. The first group received no treatment. The MLT group was treated with a concentration of 1 mM melatonin. In the CoCl₂ group, 0.2 mM CoCl₂ was added to the medium to induce Hif1α overexpression. The MLT+CoCl₂ group received 0.2 mM CoCl₂ and 1 mM melatonin. After 24 hr treatment, the cells and supernatants were collected and used for further determination. The MTT assay was performed to estimate the decrease in cell viability throughout the CoCl₂ and melatonin treatment.

SUBMITTER: Karimi S 

PROVIDER: S-EPMC10440132 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature