Cardiac ischemia on-a-chip to investigate cellular and molecular response of myocardial tissue under hypoxia.

ABSTRACT: Tissue engineering has enabled the development of advanced and physiologically relevant models of cardiovascular diseases, with advantages over conventional 2D in vitro assays. We have previously demonstrated development of a heart on-a-chip microfluidic model with mature 3D anisotropic tissue formation that incorporates both stem cell-derived cardiomyocytes and cardiac fibroblasts within a collagen-based hydrogel. Using this platform, we herein present a model of myocardial ischemia on-a-chip, that recapitulates ischemic insult through exposure of mature 3D cardiac tissues to hypoxic environments. We report extensive validation and molecular-level analyses of the model in its ability to recapitulate myocardial ischemia in response to hypoxia, demonstrating the 1) induction of tissue fibro

SUBMITTER: Veldhuizen J 

PROVIDER: S-EPMC10440189 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature