A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites.

ABSTRACT: Early and accurate detection of infection by pathogenic microorganisms, such as Plasmodium, the causative agent of malaria, is critical for clinical diagnosis and ultimately determines the patient's outcome. We have combined a polystyrene-based microfluidic device with an immunoassay which utilises Surface-Enhanced Raman Spectroscopy (SERS) to detect malaria. The method can be easily translated to a point-of-care testing format and shows excellent sensitivity and specificity, when compared to the gold standard for laboratorial detection of Plasmodium infections. The device can be fabricated in less than 30 min by direct patterning on shrinkable polystyrene sheets of adaptable three-dimensional microfluidic chips. To validate the microfluidic system, samples of P. falciparum-infected

SUBMITTER: Oliveira MJ 

PROVIDER: S-EPMC10440799 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature