MicroRNA-124 regulates Notch and NeuroD1 to mediate transition states of neuronal development.

ABSTRACT: MicroRNAs regulate gene expression by destabilizing target mRNA and/or inhibiting translation in animal cells. The ability to mechanistically dissect miR-124's function during specification, differentiation, and maturation of neurons during development within a single system has not been accomplished. Using the sea urchin embryo, we take advantage of the manipulability of the embryo and its well-documented gene regulatory networks (GRNs). We incorporated NeuroD1 as part of the sea urchin neuronal GRN and determined that miR-124 inhibition resulted in aberrant gut contractions, swimming velocity, and neuronal development. Inhibition of miR-124 resulted in an increased number of cells expressing transcription factors (TFs) associated with progenitor neurons and a concurrent decrease of matur

SUBMITTER: Konrad KD 

PROVIDER: S-EPMC10440801 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature