Immunodominant conformational and linear IgE epitopes lie in a single segment of Ara h 2.

ABSTRACT:

Background

Contribution of conformational epitopes to the IgE reactivity of peanut allergens Ara h 2 and Ara h 6 is at least as important as that of the linear epitopes. However, little is known about these conformational IgE-binding epitopes.

Objective

We investigated the distribution of conformational epitopes on chimeric 2S-albumins.

Methods

Recombinant chimeras were generated by exchanging structural segments between Ara h 2 and Ara h 6. Well-refolded chimeras, as verified by circular dichroism analysis, were then used to determine the epitope specificity of mAbs by performing competitive inhibition of IgG binding. Furthermore, we delineated the contribution of each segment to the overall IgE reactivity of both 2S-albumins by measuring the chimeras' IgE-binding ca

SUBMITTER: Hazebrouck S 

PROVIDER: S-EPMC10440805 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature