Transforming the untransformable with knockout minicircles

ABSTRACT: Abstract Gene inactivation studies are critical in pathogenic bacteria, where insights into species biology can guide the development of vaccines and treatments. Allelic exchange via homologous recombination is a generic method of targeted gene editing in bacteria. However, generally applicable protocols are lacking, and suboptimal approaches are often used for nonstandard but epidemiologically important species. Photobacterium damselae subsp. piscicida (Pdp) is a primary pathogen of fish in aquaculture and has been considered hard to transform since the mid‐1990s. Consequently, conjugative transfer of RK2/RP4 suicide vectors from Escherichia coli S17‐1/SM10 donor strains, a system prone to off‐target mutagenesis, was used to deliver the allelic exchange DNA in previous studies. Here we ha

SUBMITTER: Rudenko O 

PROVIDER: S-EPMC10441182 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature