Senescence during early differentiation reduced the chondrogenic differentiation capacity of mesenchymal progenitor cells.

ABSTRACT: Introduction: Mesenchymal stromal/progenitor cells (MSCs) are promising for cartilage cell-based therapies due to their chondrogenic differentiation capacity. However, MSCs can become senescent during in vitro expansion, a state characterized by stable cell cycle arrest, metabolic alterations, and substantial changes in the gene expression and secretory profile of the cell. In this study, we aimed to investigate how senescence and the senescence-associated secretory phenotype (SASP) affect chondrogenic differentiation of MSCs. Methods: To study the effect of senescence, we exposed MSCs to gamma irradiation during expansion or during chondrogenic differentiation (the pellet culture). Western blot analysis was used to evaluate MSCs response to the chondrogenic inductor T

SUBMITTER: Voskamp C 

PROVIDER: S-EPMC10441241 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature