Force-induced site-specific enzymatic cleavage probes reveal that serial mechanical engagement boosts T cell activation.

ABSTRACT: The surface of T cells is studded with T cell receptors (TCRs) that are used to scan target cells to identify peptide-major histocompatibility complexes (pMHCs) signatures of viral infection or cancerous mutation. It is now established that the TCR-pMHC complex is highly transient and experiences mechanical forces that augment the fidelity of T cell activation. An important question in this area pertains to the role of force duration in immune activation. Herein, we report the development of force probes that autonomously terminate tension within a time window following mechanical triggering. Force-induced site-specific enzymatic cleavage (FUSE) probes tune tension duration by controlling the rate of a force-triggered endonuclease hydrolysis reaction. This new capability provides a method

SUBMITTER: Rogers J 

PROVIDER: S-EPMC10441320 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature