Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring.

ABSTRACT: Understanding the intricate interplay and inter-connectivity of biological processes across an entire organism is important in various fields of biology, including cardiovascular research, neuroscience, and developmental biology. Here, we present a mesoscopic oblique plane microscope (OPM) that enables whole organism imaging with high speed and subcellular resolution. A microprism underneath the sample enhances the axial resolution and optical sectioning through total internal reflection of the light-sheet. Through rapid refocusing of the light-sheet, the imaging depth is extended up to threefold while keeping the axial resolution constant. Using low magnification objectives with a large field of view, we realize mesoscopic imaging over a volume of 3.7×1.5×1 mm³ with ~2.3 micron

SUBMITTER: Daetwyler S 

PROVIDER: S-EPMC10441428 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature