Substrate topographies modulate the secretory activity of human bone marrow mesenchymal stem cells.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) secrete a diversity of factors with broad therapeutic potential, yet current culture methods limit potency outcomes. In this study, we used topographical cues on polystyrene films to investigate their impact on the secretory profile and potency of bone marrow-derived MSCs (hBM-MSCs). hBM-MSCs from four donors were cultured on topographic substrates depicting defined roughness, curvature, grooves and various levels of wettability.

Methods

The topographical PS-based array was developed using razor printing, polishing and plasma treatment methods. hBM-MSCs from four donors were purchased from RoosterBio and used in co-culture with peripheral blood mononuclear cells (PBMCs) from Cell Applications Inc. in an immunopotency assay to measure

SUBMITTER: Rosado-Galindo H 

PROVIDER: S-EPMC10441765 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature