Gain-of-function mutations of AtNHX1 suppress sos1 salt sensitivity and improve salt tolerance in Arabidopsis.

ABSTRACT: Soil salinity severely hampers agricultural productivity. Under salt stress, excess Na⁺ accumulation causes cellular damage and plant growth retardation, and membrane Na⁺ transporters play central roles in Na⁺ uptake and exclusion to mitigate these adverse effects. In this study, we performed sos1 suppressor mutant (named sup) screening to uncover potential genetic interactors of SOS1 and additional salt tolerance mechanisms. Map-based cloning and sequencing identified a group of mutants harboring dominant gain-of-function mutations in the vacuolar Na⁺/H⁺ antiporter gene AtNHX1. The gain-of-function variants of AtNHX1 showed enhanced transporter activities in yeast cells and increased salt tolerance in Arabidopsis wild type plants. Ion

SUBMITTER: Pabuayon ICM 

PROVIDER: S-EPMC10441915 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature