Turnip mosaic virus manipulates DRM2 expression to regulate host CHH and CHG methylation for robust infection.

ABSTRACT: DNA methylation is an important epigenetic marker for the suppression of transposable elements (TEs) and the regulation of plant immunity. However, little is known how RNA viruses counter defense such antiviral machinery. In this study, the change of DNA methylation in turnip mosaic virus (TuMV)-infected cells was analyzed by whole genome bisulfite sequencing. Results showed that the total number of methylated sites of CHH and CHG increased in TuMV-infected cells, the majority of differentially methylated regions (DMRs) in the CHH and CHG contexts were associated with hypermethylation. Gene expression analysis showed that the expression of two methylases (DRM2 and CMT3) and three demethylases (ROS3, DML2, DML3) was significantly increased and decreased in TuMV-infected cells, respectively.

SUBMITTER: Wu X 

PROVIDER: S-EPMC10441925 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature