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Hydrogen peroxide mediates high-intensity blue light-induced hypocotyl phototropism of cotton seedlings.

ABSTRACT: Phototropism is a classic adaptive growth response that helps plants to enhance light capture for photosynthesis. It was shown that hydrogen peroxide (H₂O₂) participates in the regulation of blue light-induced hypocotyl phototropism; however, the underlying mechanism is unclear. In this study, we demonstrate that the unilateral high-intensity blue light (HBL) could induce asymmetric distribution of H₂O₂ in cotton hypocotyls. Disruption of the HBL-induced asymmetric distribution of H₂O₂ by applying either H₂O₂ itself evenly on the hypocotyls or H₂O₂ scavengers on the lit side of hypocotyls could efficiently inhibit hypocotyl phototropic growth. Consistently, application of H₂O₂ on the shaded and lit sides of the hypocotyls led to reduced and enhanced hypocotyl phototropism, respectively. Further, we show that H₂O₂ inhibits hypocotyl elongation of cotton seedlings, thus supporting the repressive role of H₂O₂ in HBL-induced hypocotyl phototropism. Moreover, our results show that H₂O₂ interferes with HBL-induced asymmetric distribution of auxin in the cotton hypocotyls. Taken together, our study uncovers that H₂O₂ changes the asymmetric accumulation of auxin and inhibits hypocotyl cell elongation, thus mediating HBL-induced hypocotyl phototropism.

SUBMITTER: Lv QY

PROVIDER: S-EPMC10442013 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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Hydrogen peroxide mediates high-intensity blue light-induced hypocotyl phototropism of cotton seedlings.

Lv Qian-Yi QY Zhao Qing-Ping QP Zhu Chen C Ding Meichen M Chu Fang-Yuan FY Li Xing-Kun XK Cheng Kai K Zhao Xiang X

Stress biology 20230726 1

Phototropism is a classic adaptive growth response that helps plants to enhance light capture for photosynthesis. It was shown that hydrogen peroxide (H2O2) participates in the regulation of blue light-induced hypocotyl phototropism; however, the underlying mechanism is unclear. In this study, we demonstrate that the unilateral high-intensity blue light (HBL) could induce asymmetric distribution of H2O2 in cotton hypocotyls. Disruption of the HBL-induc ...[more]

PMID: 37676397

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Project description:Soybean sprouts are a flavorful microgreen that can be eaten all year round and are widely favored in Southeast Asia. In this study, the regulatory mechanism of calcium on anthocyanin biosynthesis in soybean sprouts under blue light was investigated. The results showed that blue light, with a short wavelength, effectively induced anthocyanin accumulation in the hypocotyl of soybean sprout cultivar "Dongnong 690." Calcium supplementation further enhanced anthocyanin content, which was obviously inhibited by LaCl3 and neomycin treatment. Moreover, exogenous calcium changed the metabolism of anthocyanins, and seven anthocyanin compounds were detected. The trend of calcium fluorescence intensity in hypocotyl cells, as well as that of the inositol 1,4,5-trisphosphate and calmodulin content, was consistent with that of anthocyanins content. Specific spatial distribution patterns of calcium antimonate precipitation were observed in the ultrastructure of hypocotyl cells under different conditions. Furthermore, calcium application upregulated the expression of genes related to anthocyanin biosynthesis, and calcium inhibitors suppressed these genes. Finally, transcriptomics was performed to gain global insights into the molecular regulation mechanism of calcium-associated anthocyanin production. Genes from the flavonoid biosynthesis pathway were distinctly enriched among the differentially expressed genes, and weighted gene co-expression network analysis showed that two MYBs were related to the accumulation of anthocyanins. These results indicated that calcium released from apoplast and intracellular stores in specific spatial-temporal features promote blue light-induced anthocyanin accumulation by upregulation of the expression of genes related to anthocyanin synthesis of "Dongnong 690" hypocotyl. The findings deepen the understanding of the calcium regulation mechanism of blue light-induced anthocyanin accumulation in soybean sprouts, which will help growers produce high-quality foods beneficial for human health.

Dataset Information

Hydrogen peroxide mediates high-intensity blue light-induced hypocotyl phototropism of cotton seedlings.

Publications

Hydrogen peroxide mediates high-intensity blue light-induced hypocotyl phototropism of cotton seedlings.

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