A three-colour stress biosensor reveals multimodal response in single cells and spatiotemporal dynamics of biofilms.

ABSTRACT: The plethora of stress factors that can damage microbial cells has evolved sophisticated stress response mechanisms. While existing bioreporters can monitor individual responses, sensors for detecting multimodal stress responses in living microorganisms are still lacking. Orthogonally detectable red, green, and blue fluorescent proteins combined in a single plasmid, dubbed RGB-S reporter, enable simultaneous, independent, and real-time analysis of the transcriptional response of Escherichia coli using three promoters which report physiological stress (PosmY for RpoS), genotoxicity (PsulA for SOS), and cytotoxicity (PgrpE for RpoH). The bioreporter is compatible with standard analysis and Fluorescent Activated Cell Sorting (FACS) combined with subsequent transcriptome analysis. Various stre

SUBMITTER: Zoheir AE 

PROVIDER: S-EPMC10442448 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature