Culture of Primary Neurons from Dissociated and Cryopreserved Mouse Trigeminal Ganglion.

ABSTRACT: Corneal nerves originate from the ophthalmic branch of the trigeminal nerve, which enters the cornea at the limbus radially from all directions toward the central cornea. The cell bodies of the sensory neurons of trigeminal nerve are located in the trigeminal ganglion (TG), while the axons are extended into the three divisions, including ophthalmic branch that supplies corneal nerves. Study of primary neuronal cultures established from the TG fibers can therefore provide a knowledge basis for corneal nerve biology and potentially be developed as an in vitro platform for drug testing. However, setting up primary neuron cultures from animal TG has been dubious with inconsistency among laboratories due to a lack of efficient isolation protocol, resulting in low yield and heterogenous c

SUBMITTER: Lin MT 

PROVIDER: S-EPMC10442681 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature