Multiplicity of the Agrobacterium Infection of Nicotiana benthamiana for Transient DNA Delivery.

ABSTRACT: Biological DNA transfer into plant cells mediated by Agrobacterium represents one of the most powerful tools for the engineering and study of plant systems. Transient expression of transfer DNA (T-DNA) in particular enables rapid testing of gene products and has been harnessed for facile combinatorial expression of multiple genes. In analogous mammalian cell-based gene expression systems, a clear sense of the multiplicity of infection (MOI) allows users to predict and control viral transfection frequencies for applications requiring single versus multiple transfection events per cell. Despite the value of Agrobacterium-mediated transient transformation of plants, MOI has not been quantified. Here, we analyze the Poisson probability distribution of the T-DNA transfer in leaf p

SUBMITTER: Carlson ED 

PROVIDER: S-EPMC10443027 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature