Combinatorial extracellular matrix cues with mechanical strain induce differential effects on myogenesis in vitro.

ABSTRACT: Skeletal muscle regeneration remains a clinical unmet need for volumetric muscle loss and atrophy where muscle function cannot be restored to prior capacity. Current experimental approaches do not account for the complex microenvironmental factors that modulate myogenesis. In this study we developed a biomimetic tissue chip platform to systematically study the combined effects of the extracellular matrix (ECM) microenvironment and mechanical strain on myogenesis of murine myoblasts. Using stretchable tissue chips composed of collagen I (C), fibronectin (F) and laminin (L), as well as their combinations thereof, we tested the addition of mechanical strain regimens on myogenesis at the transcriptomic and translational levels. Our results show that ECMs have a significant effect on myotube fo

SUBMITTER: Chan AHP 

PROVIDER: S-EPMC10443049 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature