Bioluminescent Genetically Encoded Glutamate Indicators for Molecular Imaging of Neuronal Activity.

ABSTRACT: Genetically encoded optical sensors and advancements in microscopy instrumentation and techniques have revolutionized the scientific toolbox available for probing complex biological processes such as release of specific neurotransmitters. Most genetically encoded optical sensors currently used are based on fluorescence and have been highly successful tools for single-cell imaging in superficial brain regions. However, there remains a need to develop new tools for reporting neuronal activity in vivo within deeper structures without the need for hardware such as lenses or fibers to be implanted within the brain. Our approach to this problem is to replace the fluorescent elements of the existing biosensors with bioluminescent elements. This eliminates the need of external light sources

SUBMITTER: Petersen ED 

PROVIDER: S-EPMC10443529 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature