TRIM5 Promotes Systemic Lupus Erythematosus Through CD4(+) T Cells and Macrophage.

ABSTRACT:

Purpose

Systemic lupus erythematosus (SLE) is a typical autoimmune disease characterized by the involvement of multiple organs and the production of antinuclear antibodies. This study aimed to investigate the molecular mechanism of SLE.

Patients and methods

We retrieved genome-wide gene expression levels from five public datasets with relatively large sample sizes from the Gene Expression Omnibus (GEO), and we compared the expression profiles of peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy controls (HCs). The expression of seven target genes in PBMCs from 25 cases and 3 HCs was further validated by reverse-transcription quantitative PCR (RT‒qPCR). Flow cytometry was used for verifying the proportion of naive CD4(+) T cells and M2 macrophages in PB

SUBMITTER: Pan Z 

PROVIDER: S-EPMC10443694 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature