A workflow to visualize vertebrate eyes in 3D.

ABSTRACT:

Purpose

To establish a workflow to visualize the surgical anatomy in 3D based on histological data of eyes of experimental animals for improving the planning of complex surgical procedures.

Methods

Four C57BL/6J wild-type(wt) mouse eyes, three Brown Norway rat eyes and four Chinchilla Bastard rabbit eyes were enucleated and processed for standard histology with serial sections and hematoxylin and eosin staining. Image stacks were processed to obtain a representation of the eye anatomy in 3D. In addition, virtual image stacks and 3D point clouds were generated by processing sagittal sections of eyes with stepwise 180° rotation and projection around the eye axis to construct a rotationally symmetric 3D model from one single sagittal section.

Results

Serial sections of w

SUBMITTER: Wang J 

PROVIDER: S-EPMC10443858 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature