Platform-agnostic CellNet enables cross-study analysis of cell fate engineering protocols.

ABSTRACT: Optimization of cell engineering protocols requires standard, comprehensive quality metrics. We previously developed CellNet, a computational tool to quantitatively assess the transcriptional fidelity of engineered cells compared with their natural counterparts, based on bulk-derived expression profiles. However, this platform and others were limited in their ability to compare data from different sources, and no current tool makes it easy to compare new protocols with existing state-of-the-art protocols in a standardized manner. Here, we utilized our prior application of the top-scoring pair transformation to build a computational platform, platform-agnostic CellNet (PACNet), to address both shortcomings. To demonstrate the utility of PACNet, we applied it to thousands of samples from ove

SUBMITTER: Lo EKW 

PROVIDER: S-EPMC10444577 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature