Megakaryocyte NLRP3 hyperactivation induces mild anemia and potentiates inflammatory response in mice.

ABSTRACT:

Background

The NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome has been described in both immune cells and platelets, but its role in the megakaryocyte (MK) lineage remains elusive.

Objective

The aim of this study was to explore the role of NLRP3 inflammasome in megakaryocytes and platelets.

Methods

We generated Nlrp3 ^A350V/+/Gp1ba-Cre^KI/+ mice carrying a mutation genetically similar to the one observed in human Muckle-Wells syndrome, which leads to hyperactivity of NLRP3 specifically in MK and platelets.

Results

Platelets from the mutant mice expressed elevated levels of both precursor and active form of caspase-1, suggesting hyperactivity of NLRP3 inflammasome. Nlrp3 ^A350V/+/Gp1ba

SUBMITTER: Bourne JH 

PROVIDER: S-EPMC10445124 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature