SY18ΔL60L: a new recombinant live attenuated African swine fever virus with protection against homologous challenge.

ABSTRACT:

Introduction

African swine fever (ASF) is an acute and highly contagious disease and its pathogen, the African swine fever virus (ASFV), threatens the global pig industry. At present, management of ASF epidemic mainly relies on biological prevention and control methods. Moreover, due to the large genome of ASFV, only half of its genes have been characterized in terms of function.

Methods

Here, we evaluated a previously uncharacterized viral gene, L60L. To assess the function of this gene, we constructed a deletion strain (SY18ΔL60L) by knocking out the L60L gene of the SY18 strain. To evaluate the growth characteristics and safety of the SY18ΔL60L, experiments were conducted on primary macrophages and pigs, respectively.

Results

The results revealed that the growth tr

SUBMITTER: Yang J 

PROVIDER: S-EPMC10445127 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature