Chemical QuantArray: A Quantitative Tool for Mass Spectrometry Imaging.

ABSTRACT: Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is a powerful analytical technique that provides spatially preserved detection and quantification of analytes in tissue specimens. However, clinical translation still requires improved throughput, precision, and accuracy. To accomplish this, we created "Chemical QuantArray", a gelatin tissue microarray (TMA) mold filled with serial dilutions of isotopically labeled endogenous metabolite standards. The mold is then cryo-sectioned onto a tissue homogenate to produce calibration curves. To improve precision and accuracy, we automatically remove pixels outside of each TMA well and investigated several intensity normalizations, including the utilization of a second stable isotope internal standard (IS). Chemical Q

SUBMITTER: Stopka SA 

PROVIDER: S-EPMC10445330 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature