Quantitative flow cytometric selection of tau conformational nanobodies specific for pathological aggregates.

ABSTRACT: Single-domain antibodies, also known as nanobodies, are broadly important for studying the structure and conformational states of several classes of proteins, including membrane proteins, enzymes, and amyloidogenic proteins. Conformational nanobodies specific for aggregated conformations of amyloidogenic proteins are particularly needed to better target and study aggregates associated with a growing class of associated diseases, especially neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. However, there are few reported nanobodies with both conformational and sequence specificity for amyloid aggregates, especially for large and complex proteins such as the tau protein associated with Alzheimer's disease, due to difficulties in selecting nanobodies that bind to compl

SUBMITTER: Zupancic JM 

PROVIDER: S-EPMC10445546 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature