B cell receptor-induced protein dynamics and the emerging role of SUMOylation revealed by proximity proteomics.

ABSTRACT: Successful B cell activation, which is critical for high-affinity antibody production, is controlled by the B cell antigen receptor (BCR). However, we still lack a comprehensive protein-level view of the very dynamic multi-branched cellular events triggered by antigen binding. Here, we employed APEX2 proximity biotinylation to study antigen-induced changes, 5-15 min after receptor activation, at the vicinity of the plasma membrane lipid rafts, wherein BCR enriches upon activation. The data reveals dynamics of signaling proteins, as well as various players linked to the subsequent processes, such as actin cytoskeleton remodeling and endocytosis. Interestingly, our differential expression analysis identified dynamic responses in various proteins previously not linked to early B cell activati

SUBMITTER: Awoniyi LO 

PROVIDER: S-EPMC10445728 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature