Characterization of an eye field-like state during optic vesicle organoid development.

ABSTRACT: Specification of the eye field (EF) within the neural plate marks the earliest detectable stage of eye development. Experimental evidence, primarily from non-mammalian model systems, indicates that the stable formation of this group of cells requires the activation of a set of key transcription factors. This crucial event is challenging to probe in mammals and, quantitatively, little is known regarding the regulation of the transition of cells to this ocular fate. Using optic vesicle organoids to model the onset of the EF, we generate time-course transcriptomic data allowing us to identify dynamic gene expression programmes that characterize this cellular-state transition. Integrating this with chromatin accessibility data suggests a direct role of canonical EF transcription factors in reg

SUBMITTER: Owen LJ 

PROVIDER: S-EPMC10445745 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature