GBP2 promotes M1 macrophage polarization by activating the notch1 signaling pathway in diabetic nephropathy.

ABSTRACT:

Background

Diabetic nephropathy (DN) is one of the most common diabetic complications, which has become the primary cause of end-stage renal disease (ESRD) globally. Macrophage infiltration has been proven vital in the occurrence and development of DN. This study was designed to investigate the hub genes involved in macrophage-mediated inflammation of DN via bioinformatics analysis and experimental validation.

Methods

Gene microarray datasets were obtained from the Gene Expression Omnibus (GEO) public website. Integrating the CIBERSORT, weighted gene co-expression network analysis (WGCNA) and DEGs, we screened macrophage M1-associated key genes with the highest intramodular connectivity. Subsequently, the Least Absolute Shrinkage and Selection Operator (LASSO) regression was

SUBMITTER: Li X 

PROVIDER: S-EPMC10445759 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature