Three-dimensionally visualized ossification and mineralization process of the otic capsule in a postnatal developmental mouse.

ABSTRACT:

Objective

We aimed to elucidate the ossification process of the otic capsule in postnatal C57BL/6 mice and depict the three-dimensional (3D) process of otoconial mineralization in vivo.

Methods

The otic capsules of C57BL/6 mice were stained with alizarin red and imaged/compared using micro-computed tomography on postnatal day (P) between P0 and P8, P10, P15, and P30 and 3-4 months old (P3-4Mo). We reconstructed 3D images of the otic capsule and otoconia and measured the bone mineral density using x-ray absorptiometry on each age.

Results

The 3D reconstructed otic capsule images revealed two ossification centers of the otic capsule at P0. One was observed around the ampulla of the superior semicircular canal and utricle, and the other was observed around the ampulla of

SUBMITTER: Bai J 

PROVIDER: S-EPMC10446274 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature