Blocking SP/NK1R signaling improves spinal cord hemisection by inhibiting the release of pro-inflammatory cytokines in rabbits.

ABSTRACT:

Objective

Incomplete spinal cord injury (SCI) is the most common spinal cord injury in clinic, however its mechanism is still not fully understood.

Design

We constructed the rabbit spinal cord hemisection (SCH) model and used RT-PCR, western blotting, immunohistochemistry, and immunofluorescence experiments to explore the potential mechanism of SCI.

Setting

The sham operation (SH) group, the observation (OB, which is the SCH) group, the OB+ substance p (SP) inhibitor group, the OB + NK1R inhibitor group, the OB + NK1R agonist group and the OB + SP inhibitor + NK1R agonist group.

Participants

New Zealand white rabbits.

Interventions

Use NK1R inhibitors, NK1R agonists, SP inhibitors to treat the SCH model.

Outcome measures

IL-1β, IKKγ, IL-6 and NF-κ

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC10446800 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature