The crosstalk between microbiota and metabolites in AP mice: an analysis based on metagenomics and untargeted metabolomics.

ABSTRACT:

Background and purpose

Microbiome dysfunction is known to aggravate acute pancreatitis (AP); however, the relationship between this dysfunction and metabolite alterations is not fully understood. This study explored the crosstalk between the microbiome and metabolites in AP mice.

Methods

Experimental AP models were established by injecting C57/BL mice with seven doses of cerulein and one dose of lipopolysaccharide (LPS). Metagenomics and untargeted metabolomics were used to identify systemic disturbances in the microbiome and metabolites, respectively, during the progression of AP.

Results

The gut microbiome of AP mice primarily included Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria, and "core microbiota" characterized by an increase in Proteobacteria

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC10446838 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature