Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification.

ABSTRACT: Corals are under siege by both local and global threats, creating a worldwide reef crisis. Cryopreservation is an important intervention measure and a vital component of the modern coral conservation toolkit, but preservation techniques are currently limited to sensitive reproductive materials that can only be obtained a few nights per year during spawning. Here, we report the successful cryopreservation and revival of cm-scale coral fragments via mL-scale isochoric vitrification. We demonstrate coral viability at 24 h post-thaw using a calibrated oxygen-uptake respirometry technique, and further show that the method can be applied in a passive, electronics-free configuration. Finally, we detail a complete prototype coral cryopreservation pipeline, which provides a platform for essential n

SUBMITTER: Powell-Palm MJ 

PROVIDER: S-EPMC10447501 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature