Stable trapping of multiple proteins at physiological conditions using nanoscale chambers with macromolecular gates.

ABSTRACT: The possibility to detect and analyze single or few biological molecules is very important for understanding interactions and reaction mechanisms. Ideally, the molecules should be confined to a nanoscale volume so that the observation time by optical methods can be extended. However, it has proven difficult to develop reliable, non-invasive trapping techniques for biomolecules under physiological conditions. Here we present a platform for long-term tether-free (solution phase) trapping of proteins without exposing them to any field gradient forces. We show that a responsive polymer brush can make solid state nanopores switch between a fully open and a fully closed state with respect to proteins, while always allowing the passage of solvent, ions and small molecules. This makes it possible

SUBMITTER: Svirelis J 

PROVIDER: S-EPMC10447545 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature