Photocycle alteration and increased enzymatic activity in genetically modified photoactivated adenylate cyclase OaPAC.

ABSTRACT: Photoactivated adenylate cyclases (PACs) are light activated enzymes that combine blue light sensing capacity with the ability to convert ATP to cAMP and pyrophosphate (PPi) in a light-dependent manner. In most of the known PACs blue light regulation is provided by a blue light sensing domain using flavin which undergoes a structural reorganization after blue-light absorption. This minor structural change then is translated toward the C-terminal of the protein, inducing a larger conformational change that results in the ATP conversion to cAMP. As cAMP is a key second messenger in numerous signal transduction pathways regulating various cellular functions, PACs are of great interest in optogenetic studies. The optimal optogenetic device must be "silent" in the dark and highly responsive upo

SUBMITTER: Raics K 

PROVIDER: S-EPMC10448171 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature