ER stress increases expression of intracellular calcium channel RyR1 to modify Ca²⁺ homeostasis in pancreatic beta cells.

ABSTRACT: Pancreatic beta cells maintain glucose homeostasis by secreting pulses of insulin in response to a rise in plasma glucose. Pulsatile insulin secretion occurs as a result of glucose-induced oscillations in beta-cell cytosolic Ca²⁺. The endoplasmic reticulum (ER) helps regulate beta-cell cytosolic Ca²⁺, and ER stress can lead to ER Ca²⁺ reduction, beta-cell dysfunction, and an increased risk of type 2 diabetes. However, the mechanistic effects of ER stress on individual calcium channels are not well understood. To determine the effects of tunicamycin-induced ER stress on ER inositol 1,4,5-triphosphate receptors (IP3Rs) and ryanodine receptors (RyRs) and their involvement in subsequent Ca²⁺ dysregulation, we treated INS-1 832/13 cells and primary mo

SUBMITTER: Zhang IX 

PROVIDER: S-EPMC10448220 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature