Amphetamine-induced reverse transport of dopamine does not require cytosolic Ca².

ABSTRACT: Amphetamines (AMPHs) are substrates of the dopamine transporter (DAT) and reverse the direction of dopamine (DA) transport. This has been suggested to depend on activation of Ca²⁺-dependent pathways, but the mechanism underlying reverse transport via endogenously expressed DAT is still unclear. Here, to enable concurrent visualization by live imaging of extracellular DA dynamics and cytosolic Ca²⁺ levels, we employ the fluorescent Ca²⁺ sensor jRGECO1a expressed in cultured dopaminergic neurons together with the fluorescent DA sensor GRAB_DA1H expressed in cocultured "sniffer" cells. In the presence of the Na⁺-channel blocker tetrodotoxin to prevent exocytotic DA release, AMPH induced in the cultured neurons a profound dose-dependent eff

SUBMITTER: Stoier JF 

PROVIDER: S-EPMC10448275 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature