Correction for Extrinsic Background in Raman Hyperspectral Images.

ABSTRACT: Raman hyperspectral microscopy is a valuable tool in biological and biomedical imaging. Because Raman scattering is often weak in comparison to other phenomena, prevalent spectral fluctuations and contaminations have brought advancements in analytical and chemometric methods for Raman spectra. These chemometric advances have been key contributors to the applicability of Raman imaging to biological systems. As studies increase in scale, spectral contamination from extrinsic background, intensity from sources such as the optical components that are extrinsic to the sample of interest, has become an emerging issue. Although existing baseline correction schemes often reduce intrinsic background such as autofluorescence originating from the sample of interest, extrinsic background is not explic

SUBMITTER: Taylor JN 

PROVIDER: S-EPMC10448497 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature