First insights into the Aurelia aurita transcriptome response upon manipulation of its microbiome.

ABSTRACT:

Introduction

The associated diverse microbiome contributes to the overall fitness of Aurelia aurita, particularly to asexual reproduction. However, how A. aurita maintains this specific microbiome or reacts to manipulations is unknown.

Methods

In this report, the response of A. aurita to manipulations of its native microbiome was studied by a transcriptomics approach. Microbiome-manipulated polyps were generated by antibiotic treatment and challenging polyps with a non-native, native, and potentially pathogenic bacterium. Total RNA extraction followed by RNAseq resulted in over 155 million reads used for a de novo assembly.

Results

The transcriptome analysis showed that the antibiotic-induced change and resulting reduction of the microbiome

SUBMITTER: Weiland-Brauer N 

PROVIDER: S-EPMC10448538 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature