Charging of Vitreous Samples in Cryogenic Electron Microscopy Mitigated by Graphene.

ABSTRACT: Cryogenic electron microscopy can provide high-resolution reconstructions of macromolecules embedded in a thin layer of ice from which atomic models can be built de novo. However, the interaction between the ionizing electron beam and the sample results in beam-induced motion and image distortion, which limit the attainable resolutions. Sample charging is one contributing factor of beam-induced motions and image distortions, which is normally alleviated by including part of the supporting conducting film within the beam-exposed region. However, routine data collection schemes avoid strategies whereby the beam is not in contact with the supporting film, whose rationale is not fully understood. Here we characterize electrostatic charging of vitreous samples, both in imaging and in dif

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10448747 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature