Exploratory study of macrophage polarization and spatial distribution in colorectal cancer liver metastasis: a pilot study

ABSTRACT:

Background

The liver is the most typical site of metastatic disease for patients with colorectal cancer (CRC), and up to half the patients with CRC will develop colorectal liver metastasis (CLM). Studying the tumor microenvironment, particularly macrophages and their spatial distribution, can give us critical insight into treatment.

Methods

Ten CLMs (five treatment-naïve and five post–neoadjuvant chemotherapy) were stained with multiplex immunofluorescence panels against cytokeratins, CD68, Arg1, CD206, CD86, CD163, PD-L1, and MRP8-14. Densities of cell phenotypes and their spatial distribution in the tumor center and the normal liver–tumor interface were correlated with clinicopathological variables.

Results

M2 macrophages were the predominant subtype in both th

SUBMITTER: Khanduri I 

PROVIDER: S-EPMC10449458 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature